產品介紹
慢病毒包裝試劑盒
GeneCopoeia的Lenti-Pac™ HIV和FIV表達包裝系統包括優化的慢病毒包裝質粒，eGFP陽性對照質粒，轉染試劑，增加病毒產量的EndoFectin™轉染試劑和TiterBoost™滴度增強劑。該系統與GeneCopoeia的ORF、shRNA、miRNA及Promoter克隆配套使用保證更高滴度和更高表達水平。
HIV和FIV包裝系統中的包裝質粒經過設計調整，確保將重組子包裝成具有高感染率的慢病毒顆粒，同時防止了病毒的自我復制。
293Ta慢病毒包裝細胞系
293Ta包裝細胞系是常用于病毒包裝的工具細胞。Lenti-Pac™ 293Ta包裝細胞系轉染效率高，能夠實現病毒蛋白高水平的表達。
慢病毒濃縮試劑
Lenti-Pac™ 慢病毒濃縮試劑是GeneCopoeia為用戶設計的用于快速、簡便濃縮慢病毒顆粒的慢病毒濃縮試劑。實驗操作時只需將濃縮試劑與需濃縮的慢病毒上清液混合，經短暫孵育，普通離心機離心即可。
慢病毒滴度檢測試劑盒
GeneCopoeia設計的Lenti-Pac™ HIV和FIV qRT-PCR慢病毒滴度檢測試劑盒可快速、簡便地測定病毒拷貝數，并確定病毒包裝是否成功。該試劑盒主要組分包括RNA抽提試劑， qRT-PCR反應試劑以及內參對照。慢病毒的RNA基因組被反轉錄后用基于SYBR® Green I原理的qRT-PCR技術進行定量。
相關產品
- 慢病毒載體的克隆 — ORF、shRNA、miRNA、miRNA抑制劑以及Promoter克隆
- 預制慢病毒顆粒 — iPSC基因、ORF克隆、陽性和陰性對照等
- 特殊慢病毒包裝服務 — 價格低且周期短，包含ORF，miRNA或miRNA inhibitors序列的慢病毒載體
- 客戶定制的慢病毒服務 — 40多種載體和多種融合標簽，可定制任意ORF，shRNA或miRNA載體
發表的文章
- Hunter, K. et al (2013) Inherited variation in miR-290 expression suppresses breast cancer progression by targeting the metastasis susceptibility gene Arid4b. Cancer Research, doi: 10.1158/0008-5472.CAN-12-3513. [Lenti-Pac™ FIV Expression Packing Kit].
- Hirata, H. et al (2013) MicroRNA-182-5p Promotes Cell Invasion and Proliferation by Down Regulating FOXF2, RECK and MTSS1 Genes in Human Prostate Cancer. PloS one, 8(1): e55502. doi:10.1371/journal.pone.0055502. [Lenti-Pac™ HIV Expression Packing Kit].
- Sanchez-Alvarez, R. at al (2013) Mitochondrial dysfunction in breast cancer cells prevents tumor growth: Understanding chemoprevention with metformin. Cell Cycle, Volume 12, Issue 1, January 1, 2013 Pages 172-182. [Lentiviral plasmids, packaging cells and reagents].
- Musick, J. et al. (2012) Induced Pluripotent Stem Cells and Related Methods. U.S. Patent No. 20,120,276,070. 1 Nov. 2012. [Lenti-Pac™ HIV Expression Packing Kit].
- Amodio, N. et al. (2012) miR-29b sensitizes multiple myeloma cells to bortezomib-induced apoptosis through the activation of a feedback loop with the transcription factor Sp1. Cell Death and Disease, 3, e436; doi:10.1038/cddis.2012.175. [Lenti-Pac™ FIV Expression Packaging Kit].
- Simpfendorfer, K. et al. (2012) The autoimmunity-associated BLK haplotype exhibits cis-regulatory effects on mRNA and protein expression that are prominently observed in B cells early in development. Hum. Mol. Genet. (2012) 21 (17): 3918-3925. doi: 10.1093/hmg/dds220. [HIV Lenti-Pac™].
- Yamamura, S. et al. (2012) MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma. Carcinogenesis (2012) 33 (2): 294-300. doi: 10.1093/carcin/bgr286 . [HIV Lenti-Pac].
