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核酸熒光染料

DNA凝膠染料

GeneCopoeia公司最新推出了一系列核酸熒光染料產品作為EB替代品，具有安全、靈敏度高以及產品性能穩定等優點。

GreenView™, GreenView™ Plus, GreenView™ Ultra, and RedView™是GeneCopoeia公司最新研發具有安全無毒，靈敏度高，穩定性好的EB替代品, 使用方法同EB，可用于前染法和后染法，條帶整齊不彎曲。GreenView™, GreenView™ Plus, and GreenView™ Ultra可在紫外燈或藍光儀下觀察發綠色熒光；RedView™可在紫外燈下觀察發紅色熒光。

核酸電泳是核酸研究的重要手段之一，其中溴化乙啶（EB）因其低廉的價格是分子生物學中使用最早和最為廣泛的核酸電泳染色試劑。但是EB是一種強誘變劑，具有高致癌性，其廢棄液必須進行凈化處理，而且EB的檢測靈敏度有限以及背景信號較高，目前正逐漸被靈敏度高以及安全可靠的新型核酸熒光染料所取代。

產品特點

  • 安全無毒，無致突變
  • 穩定性好
  • 靈敏度高，條帶整齊

 

Green_RedView Figure 1

Figure 1. Gel stain results with GreenView™, GreenView™ Plus, and GreenView™ Ultra (Left), and comparison results with SYBR Safe™ and GelGreen™ (Right). Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with GreenView™, GreenView™ Plus, GreenView™ Ultra, SYBR Safe™ and GelGreen™, respectively. Results show GreenView™ has similar sensitivity with SYBR Safe™ and GelGreen™, GreenView™ Plus and GreenView™ Ultra have much better sensitivity.

Green_RedView Figure 1

Figure 2. Gel stain results with RedView™, and GelRed™. Two-fold serial dilutions of a 1 kb DNA ladder (NEB, Cat # N0468S) were loaded onto each gel in 3 lanes in the amounts of 25 ng, 50 ng and 100 ng (left to right), then post-stained with RedView™, and GelRed™, respectively. Results show RedView™ has similar sensitivity with GelRed™.

此外，GreenView™, GreenView™ Plus, and RedView™ 具有較低的細胞膜通透性，不易與細胞內DNA結合，安全性優于SYBR Safe。

Green_RedView Figure 2

Figure 3. Cell permeability tests with SYBR Safe, GreenView, GreenView Plus, and RedView. Hela cells were incubated at 37℃ with 1X SYBR Safe, GreenView, GreenView Plus, and RedView for 30 min. GreenView Plus and RedView could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe, which rapidly entered cells and stained nuclei.

DNA上樣染料

SafeGreen™和SafeRed™是GeneCopoeia最新推出的安全靈敏，操作簡單的次世代核酸電泳染料，電泳前只需將染料與DNA混合，上樣，即可獲得條帶清晰的染膠效果，整個過程無需前染和后染。

產品特點

  • 即用型，操作簡單
  • 安全無毒
  • 靈敏度高
  • 成本低

SafeGreen™ 和 SafeRed™ Loading Dye 含 6 × DNA 上樣緩沖液中及兩類常用的示蹤染料 （Xylene Cyanol FF 和 Bromophenol Blue），用以監測電泳期間瓊脂糖凝膠條帶（呈現紫色和藍色兩個條帶）遷移情況。DNA條帶可通過 LED 或紫外光直接觀察，無需前染和后染步驟。

Green_RedView Figure 4

Figure 4. Gel staining results with SafeGreen™ and SafeRed™ premixed with DNA ladder. 1 kb DNA Ladder (Cat# D001) was mixed with SafeGreen™ or SafeRed™ at a 5:1 ratio, then loaded onto each well. Gels were directly imaged on a UV transilluminator.

SafeGreen™ 和 SafeRed™ 無法穿透細胞膜進入活細胞，從而降低了細胞毒性(Figure 5)。

Green_RedView Figure 5

Figure 5. Cell permeability tests with SYBR Safe™, SafeGreen™, and SafeRed™. Hela cells were incubated at 37℃ with 1X SYBR Safe™, SafeGreen™, and SafeRed™ for 30 min. SafeGreen™ and SafeRed™ could not penetrate cell membranes to bind DNA in living cells, and were much safer than SYBR Safe™, which rapidly entered cells and stained nuclei.

訂購

核酸熒光染料選購指南

  GreenView GreenView Plus GreenView Ultra RedView
靈敏度 (dsDNA) Sensitive
(~2 ng)		 Highly sensitive
(~0.2 ng)		 Ultra sensitive
(~60 pg)		 Sensitive
(~1 ng)
安全性 √ √ √ √
Gel electrophoresis √ √ √ √
dsDNA √ √ √ √
ssDNA   √ √  
RNA   √ √  
前染法 √ √   √
后染法     √  
膠回收 √ √ √  
激發光源 藍光/紫外 藍光/紫外 藍光/紫外 紫外
濾光片 SYBR filter SYBR filter SYBR filter EB filter

 

核酸電泳熒光染料

購買 貨號 產品名稱 描述 規格 價格（¥）
N100 GreenView DNA Gel Stain 無毒、無突變的DNA凝膠染料 500 µl  400 
N101 GreenView Plus DNA Gel Stain 無毒、無突變的DNA凝膠染料 500 µl  600 
N103 GreenView Ultra DNA Gel Stain 無毒、無突變的DNA凝膠染料 500 µl  800 
N102 RedView DNA Gel Stain 無毒、無突變的DNA凝膠染料 500 µl  600 
D012 SafeGreen Loading Dye 無毒、無突變的DNA凝膠染料 1 mL 300
D013 SafeRed Loading Dye 無毒、無突變的DNA凝膠染料 1 mL 300
D001 1kb DNA Ladder DNA ladder 250 µl 200
D002 1kb Plus DNA Ladder DNA ladder 250 µl 270
D003 100bp Plus DNA Ladder DNA ladder 250 µl 270
D016 DNA Loading Buffer (6X) DNA 上樣緩沖液 5 ml 70