高通量基因表達差異定量檢測陣列
ExProfile™ Gene qPCR Arrays專為檢測不同組織或細胞中特定基因的表達量而設計,這些基因按照目錄產品或定制要求成套設置,可實現精確定量檢測多個編碼基因的表達水平,其檢測結果所顯示的表達差異為科研人員研究他們感興趣的疾病、信號通路的相關編碼基因提供了進一步的研究線索。對于目錄產品,每塊96孔板包含84對PCR引物(384孔板包含360對PCR引物),在同一塊96孔(或384孔)板上,設置有12個(384孔板有 24個)反應孔包被了不同類型的對照,用以檢測從反轉錄到qPCR整個過程的反應效率。
ExProfile gene qPCR array中使用的每一對特異引物都經實驗驗證,融解曲線均呈單一銳鋒,電泳條帶均呈單一條帶,且與擴增產物相符。由10種組織混合抽提的RNA經反轉錄得到的cDNA,作為引物驗證的模板。
使用通用的實時定量反應條件,即可簡便進行高通量的基因表達量檢測和分析。逆轉錄試劑All-in-One™ First-Strand cDNA Synthesis Kit(AORT-0020、AORT-0050)和以熒光染料SYBR® Green為基礎的定量檢測試劑All-in-One™ qPCR Mix(AOPR-0200、 AOPR-1000、AOPR-4000)作為被推薦的RT-qPCR試劑產品可與ExProfile™ Gene qPCR Array配套使用。該系列試劑均經過優化,具有高的靈敏度、效率和特異性。
Gene qPCR Array 系列產品
ExProfile™ Pathway qPCR arrays – 61種信號通路相關基因表達差異定量檢測陣列
ExProfile™ Cancer Gene qPCR array – 21種不同癌癥(腫瘤)相關基因表達差異定量檢測陣列
ExProfile™ disease and gene group qPCR arrays – 17種疾病(或特殊的基因功能群體)相關基因表達差異定量檢測陣列
ExProfile™ Custom Gene qPCR Array – 客戶定制基因表達量差異檢測陣列
產品優勢
驗證引物
- 每對特定基因引物均使用專利算法設計并經過實驗確證
性能優越
- 靈敏度高 — 與推薦試劑配套使用最低可檢測到4個mRNA分子
- 線性范圍寬 — 可同時檢測到不同表達水平的mRNA
- 重復性好 —批間和批內重復性好(R2 > 0.99)
目錄產品與客戶定制兼容
- 癌癥基因組群目錄產品
- 信號通路基因組群目錄產品
- 客戶定制基因組群產品
圖 1. Gene qPCR array 實驗流程
GeneCopoeia 提供各種癌癥(腫瘤)、信號通路相關基因表達量定量檢測陣列的目錄產品,同時,也提供客戶定制服務。
貨號 | 產品名稱 | 種屬 | genes個數 | 平板數 | 文檔 |
QG001 | ExProfile™ Human Autophagy Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG002 | ExProfile™ Human Angiogenesis Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG003 | ExProfile™ Human Antigen Processing & Presentation Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG004 | ExProfile™ Human AMPK Signaling Related Gene qPCR Array | Human | 336 | 4 x 96-well |
Datasheet Primer list |
QG005 | ExProfile™ Human Breast Cancer & Estrogen Receptor Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG006 | ExProfile™ Human Cell Cycle Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG007 | ExProfile™ Human Cancer Drug Resistance & Metabolism Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG008 | ExProfile™ Human Cytokine Receptor Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG009 | ExProfile™ Human cAMP/Ca2+ Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
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QG010 | ExProfile™ Human Dendritic & Antigen Presenting Cell Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG011 | ExProfile™ Human Drug Metabolism Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG012 | ExProfile™ Human DNA Damage and Repair Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG013 | ExProfile™ Human Extracellular Matrix and Adhesion Molecules Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG014 | ExProfile™ Human Electron Transport Toxicology Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG015 | ExProfile™ Human EGF/PDGF Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG016 | ExProfile™ Human Embryonic Stem Cells Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG017 | ExProfile™ Human Endothelial Cell Biology Related Gene qPCR Array | Human | 84 | 1 x 96-well |
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QG018 | ExProfile™ Human FoxP3 Target Genes qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG019 | ExProfile™ Human Angiogenic Growth Factors & Angiogenesis Inhibitors Related Gene qPCR Array Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG020 | ExProfile™ Human General Toxicology Related Gene qPCR Array | Human | 336 | 4 x 96-well |
Datasheet Primer list |
QG021 | ExProfile™ Human Growth and Development Toxicity Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG022 | ExProfile™ Human Hedgehog Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
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QG023 | ExProfile™ Human HIV Infection and Host Response Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG024 | ExProfile™ Human Insulin Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG025 | ExProfile™ Human Hematopoietic Stem Cells and Hematopoiesis Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG026 | ExProfile™ Human Hypoxia Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG027 | ExProfile™ Human Innate & Adaptive Immune Response Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG028 | ExProfile™ Human Inflammatory Cytokines & Receptors Relayed Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG029 | ExProfile™ Human Interferon Signaling & Response Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG030 | ExProfile™ Human Innate Immune Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
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QG031 | ExProfile™ Human Immunology Related Gene qPCR Array | Human | 336 | 4 x 96-well |
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QG032 | ExProfile™ Human Interferon Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG033 | ExProfile™ Human Immune Complement Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG034 | ExProfile™ Human JAK/STAT Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG035 | ExProfile™ Human NFΚΒ Signaling Pathway Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG036 | ExProfile™ Human Lipoprotein Signaling & Cholesterol Metabolism Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG037 | ExProfile™ Human Tumor Necrosis Factor (TNF) Ligand and Receptor Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG038 | ExProfile™ Human MAP Kinase Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG039 | ExProfile™ Human Neuroscience Ion Channels & Transporters Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG040 | ExProfile™ Human Nuclear Receptors and Coregulators Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG041 | ExProfile™ Human Nitric Oxide Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG042 | ExProfile™ Human Neurogenesis and Neural Stem Cell Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG043 | ExProfile™ Human Notch Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG044 | ExProfile™ Human Neurotransmitter Receptors and Regulators Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG045 | ExProfile™ Human Oxidative Stress and Antioxidant DefenseRelated Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG046 | ExProfile™ Human Osteogenesis Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG047 | ExProfile™ Human PI3K-AKT Signaling Realted Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG048 | ExProfile™ Human Inflammatory Response and Autoimmunity Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG049 | ExProfile™ Human Stem Cell Related Gene qPCR Array | Human | 420 | 5 x 96-well |
Datasheet Primer list |
QG050 | ExProfile™ Human Stress & Toxicity Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG051 | ExProfile™ Human Signal Transduction Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG052 | ExProfile™ Human T-cell and B-cell Activation Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG053 | ExProfile™ Human Cell Cycle Toxicity and Cancer Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG054 | ExProfile™ Human TGF-β Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG055 | ExProfile™ Human Th1-Th2-Th3 Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG056 | ExProfile™ Human Tumor Metastasis Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG057 | ExProfile™ Human T Cell Anergy & Immune Tolerance Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG058 | ExProfile™ Human T helper 17 (Th17) Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG059 | ExProfile™ Human Ubiquitination (Ubiquitylation) Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG060 | ExProfile™ Human Targets of Wnt/?-catenin Signaling Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG099 | ExProfile™ Human Apoptosis Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
貨號 | 產品名稱 | 種屬 | genes個數 | 平板數 | 文檔 |
QG061 | Exprofile™ Human Adenocarcinoma Gene qPCR Array | Human | 168 | 2 x 96-well plates |
Datasheet Primer list |
QG062 | Exprofile™ Human Brain Cancer Gene qPCR Array | Human | 252 | 3 x 96-well plates |
Datasheet Primer list |
QG063 | Exprofile™ Human Breast Cancer Gene qPCR Array | Human | 504 | 6 x 96-well plates |
Datasheet Primer list |
QG064 | Exprofile™ Human Bladder Cancer Gene qPCR Array | Human | 420 | 5 x 96-well plates |
Datasheet Primer list |
QG065 | Exprofile™ Human Colorectal Cancer Gene qPCR Arrays | Human | 336 | 4 x 96-well plates |
Datasheet Primer list |
QG066 | Exprofile™ Human Cervical Cancer Gene qPCR Arrays | Human | 84 | 1 x 96-well plates |
Datasheet Primer list |
QG067 | Exprofile™ Human Endometrial Cancer Gene qPCR Arrays | Human | 82 | 1 x 96-well plates |
Datasheet Primer list |
QG068 | Exprofile™ Human Head and Neck Cancer Gene qPCR Arrays | Human | 504 | 6 x 96-well plates |
Datasheet Primer list |
QG069 | Exprofile™ Human Kidney Cancer Gene qPCR Arrays | Human | 84 | 1 x 96-well plates |
Datasheet Primer list |
QG070 | Exprofile™ Human Leukemia Gene qPCR Arrays | Human | 504 | 6 x 96-well plates |
Datasheet Primer list |
QG071 | Exprofile™ Human Lung Cancer Gene qPCR Arrays | Human | 504 | 6 x 96-well plates |
Datasheet Primer list |
QG072 | Exprofile™ Human Liver Cancer Gene qPCR Arrays | Human | 168 | 2 x 96-well plates |
Datasheet Primer list |
QG073 | Exprofile™ Human Lymphoma Gene qPCR Arrays | Human | 420 | 5 x 96-well plates |
Datasheet Primer list |
QG074 | Exprofile™ Human Myeloma Gene qPCR Arrays | Human | 84 | 1 x 96-well plates |
Datasheet Primer list |
QG075 | Exprofile™ Human Ovarian Cancer Gene qPCR Arrays | Human | 336 | 4 x 96-well plates |
Datasheet Primer list |
QG076 | Exprofile™ Human Pancreatic Cancer Gene qPCR Arrays | Human | 168 | 2 x 96-well plates |
Datasheet Primer list |
QG077 | Exprofile™ Human Prostate Cancer Gene qPCR Arrays | Human | 412 | 5 x 96-well plates |
Datasheet Primer list |
QG078 | Exprofile™ Human Skin Cancer Gene qPCR Arrays | Human | 252 | 3 x 96-well plates |
Datasheet Primer list |
QG079 | Exprofile™ Human Stomach Cancer Gene qPCR Arrays | Human | 168 | 2 x 96-well plates |
Datasheet Primer list |
QG080 | Exprofile™ Human Thyroid Cancer Gene qPCR Arrays | Human | 84 | 1 x 96-well plates |
Datasheet Primer list |
QG081 | Exprofile™ Human Testicular Cancer Gene qPCR Arrays | Human | 68 | 1 x 96-well plates |
Datasheet Primer list |
貨號 | 產品名稱 | 種屬 | genes個數 | 平板數 | 文檔 |
QG082 | ExProfile™ Human Huntingtons Disease Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG083 | ExProfile™ Human Mood Disorder Related Gene qPCR Array | Human | 336 | 4 x 96-well |
Datasheet Primer list |
QG084 | ExProfile™ Human Neurodegeneration Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG085 | ExProfile™ Human Blood Coagulation Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG086 | ExProfile™ Human Chemokines & Receptors Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG087 | ExProfile™ Human Drug Transporters Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG088 | ExProfile™ Human Growth Factor Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG089 | ExProfile™ Human Histone Modification Enzyme Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG090 | ExProfile™ Human Heat Shock Proteins Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG091 | ExProfile™ Human Ion Channels Related Gene qPCR Array | Human | 336 | 4 x 96-well |
Datasheet Primer list |
QG092 | ExProfile™ Human Nuclear Hormone Receptors Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG093 | ExProfile™ Human Neurotrophin and Receptors Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
QG094 | ExProfile™ Human Protein Phosphatases Related Gene qPCR Array | Human | 168 | 2 x 96-well |
Datasheet Primer list |
QG095 | ExProfile™ Human Protein Kinases Related Gene qPCR Array | Human | 504 | 6 x 96-well |
Datasheet Primer list |
QG096 | ExProfile™ Human Proteases Related Gene qPCR Array | Human | 504 | 6 x 96-well |
Datasheet Primer list |
QG097 | ExProfile™ Human Surface Antigens (CD) Related Gene qPCR Array | Human | 252 | 3 x 96-well |
Datasheet Primer list |
QG098 | ExProfile™ Human Cell Surface Markers Related Gene qPCR Array | Human | 84 | 1 x 96-well |
Datasheet Primer list |
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ExProfile™ Custom Gene qPCR Arrays
客戶定制基因表達量差異定量檢測陣列
ExProfile™ Custom Gene qPCR Arrays 特為定量檢測客戶指定基因的表達量差異而設置。每對特定基因引物均使用專利算法設計并經過實驗確證。根據檢測基因數量和重復次數,GeneCopoeia可提供6種96孔板和8種384孔板的排布方式供客戶選擇。定制產品同樣包含各種不同種類的對照,用以監測樣品質量、RT和PCR反應效率、復孔的重復性等。
- Genomic DNA control (GDC): 檢測基因組DNA污染
- Spike-in reverse transcription control (RT): 監測反轉錄反應效率
- Positive PCR control (PCR): 驗證PCR反應效率
- Housekeeping genes (HK): 作為數據標準化過程中樣品的陽性對照(內參基因)
為達到Array產品的最佳性能,推薦使用配套試劑All-in-One™ First Strand cDNA Synthesis Kit和All-in-One™ qPCR Mix。
產品優勢 |
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驗證引物
- 每對特定基因引物均使用專利算法設計并經過實驗確證
靈活設計
- 提供6種96孔板和8種384孔板的排布方式供客戶選擇
優越性能
- 靈敏度高 — 與推薦試劑配套使用最低可檢測到4個RNA分子
- 線性范圍寬 — 可同時檢測到不同表達水平的mRNA
- 重復性好 — 板與板之間(批內)重復性達0.99以上
貨號 | 產品名稱 | 種屬 | genes個數 | 平板數 | 文檔 |
PAG-CS | Exprofile™ Gene qPCR Arrays Custom Services | N/A | N/A | Variable | Variable |
產品性能
GeneCopoeia 為各種目錄產品或定制基因Array產品提供了方便的數據分析工具。對于各種目錄產品,可根據產品名稱和產品編號,在下表中選擇并下載對應的分析工具文件。對于定制產品,可使用在線數據分析工具。
目錄產品數據分析工具
請下載目錄產品數據分析工具的應用實例,查看如何填寫和分析實驗數據
定制產品數據分析工具
- ExProfile™ Human Cancer Gene qPCR Arrays
- ExProfile™ Pathway Gene qPCR Array
- ExProfile™ Gene qPCR Array Custom Services
- All-in-One™ First-Strand cDNA Synthesis Kit
- All-in-One™ qPCR Mix
- All-in-One™ Validated Gene Primers
- OmicsLink™ Expression-Ready ORF cDNA Clones
- ORFEXPRESS™ Gateway® PLUS Shuttle Clones
- Lentifect™ ORF cDNA Lentiviral Particles